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Index > Protein center > Znf335(Gene name) > Rat
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  • Znf335 (Gene name),
  • Zinc finger protein 335 (Protein name ),  ZN335_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Znf335(Zfp335);
    Protein name:
    Zinc finger protein 335;
    Alternative:
    NRC-interacting factor 1(NIF-1);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Interacts with NCOA6; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors (By similarity). Interacts with CNOT6 (By similarity). Interacts with RQCD1; the interaction is direct (By similarity). Interacts with members of histone H3'Lys4'(H3K4) methyltransferase complexes ASCL2, CXXC1, KMT2A/MLL1, RBBP5, SETD1A and WDR5 (By similarity). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, TUBB2A, RBBP5, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity.
    Function:
    Component or associated component of some histone methyltransferase complexes may regulate transcription through recruitment of those complexes on gene promoters. Enhances ligand-dependent transcriptional activation by nuclear hormone receptors. Plays an important role in neural progenitor cell proliferation and self-renewal through the regulation of specific genes involved brain development, including REST. Also controls the expression of genes involved in somatic development and regulates, for instance, lymphoblast proliferation.
    Subcellular Location:
    Nucleus
    Protein Attributes:
    Sequence length:
    1336
    Sequence:
    50:
    MEENEVESSS | DAAPRPGQPE | EPSESGLGVG | TSEAVSADST | DAATAPGLTE | 
    100:
    ADDSGVGQSS | DSGSRSVEEV | SESISTEPLP | QGYLPDSSSV | SRGPVAEVPG | 
    150:
    GPPALVHSSA | LPDPSMLVSD | CTASSSDLGS | AIDKIIESTI | GPDLIQSCIT | 
    200:
    VTSGEEGGAE | TTQYLILQGP | DDGAPMASSM | STSTLANSLA | AIEALADGPT | 
    250:
    STSTCLEPAE | QPPGEPSSLA | QPPAPVVEEL | DLQGLEAMME | VVVVQQFKCK | 
    300:
    MCQYRSSTKA | TLLRHMRERH | FRPALAVAAA | AAGKRGRVRK | WGTSTKTTEE | 
    350:
    EGPEEEEEDD | DIVDAGAIDD | LEEDSDYNPA | EDEPRGRQLR | LQRPTPSTLR | 
    400:
    PRRRPGRPRK | LPRLETSDLH | DGIGEPLVSS | QSTQSPPELQ | DLEAPSSSDL | 
    450:
    RALGKVGRGL | VETGVSQSDA | ENAAPSCQDE | ADVPPRRRGR | PSRRFLGKKY | 
    500:
    RKYYYKSPKP | LLRPYLCRIC | GSRFLSHEDL | RFHVNSHEAG | DPQLFKCLQC | 
    550:
    SYRSRRWSSL | KEHMFNHVGS | KPYKCDECSY | TSVYRKDVIR | HAAVHSQDRK | 
    600:
    KRPDPTPKLS | SFPCPVCGRV | YPMQKRLTQH | MKTHSTEKPH | MCDKCGKSFK | 
    650:
    KRYTFKMHLL | THIQAVANRR | FKCEFCEFVC | EDKKALLNHQ | LSHVSDKPFK | 
    700:
    CSFCPYRTFR | EDFLLSHVAV | KHTGAKPFAC | EYCHFSTRHK | KNLRLHVRCR | 
    750:
    HANSFEEWGR | RHPEEPPSRR | RPFFSLQQIE | ELKQQHSAAP | GPPLSSAGPE | 
    800:
    APQEPAPFQP | PETPPLLCPD | ALGGATIIYQ | QGAEESTAMA | TQTALDLLLN | 
    850:
    MSAQRELGAT | ALQVAVVKSE | DVEAELTSTA | RQPSSEDTTP | RVVTLHVAES | 
    900:
    GSSVAAESQL | GPSDLQQIAL | PPGPFSGASY | SVITAPPVEG | RASASGPPYR | 
    950:
    EEPPGEAAQA | VVVNDTLKEA | GTHYIMAADG | TQLHHIELTA | DGSISFPSPD | 
    1000:
    TLAPGTKWPL | LQCGGPPRDG | PEVLSPTKTH | HTGGSQGSST | PPPATSHALG | 
    1050:
    LLVPHSPPSA | AASSTKKFSC | KVCSEAFPSR | AEMESHKRAH | AGPAAFKCPD | 
    1100:
    CPFSARQWPE | VRAHMAQHSS | LRPHQCNQCS | FASKNKKDLR | RHMLTHTNEK | 
    1150:
    PFSCHVCGQR | FNRNGHLKFH | IQRLHSIDGR | KTGTSTARAP | AQTIILNSEE | 
    1200:
    ETLATLHTAF | QSNHGTLGTE | RLQQALSQEH | IIVAQEQTVA | NQEEATYIQE | 
    1250:
    ITADGQTVQH | LVTSDNQVQY | IISQDGVQHL | LPQEYVVVPD | GHHIQVQEGQ | 
    1300:
    ITHIQYEQGT | PFLQESQIQY | VPVSPSQQLV | TQAQLEAAAH | SAVTAVADAA | 
    1336:
    MAQAQGLFGT | EEAVPEHIQQ | LQHQGIEYDV | ITLSDD
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Zinc finger protein 335
    Cat.:
    E1019h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Zinc finger protein 335
    Cat.:
    E1019m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Zinc finger protein 335
    Cat.:
    E1019r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Zinc finger protein 335
    Cat.:
    U1019h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Zinc finger protein 335
    Cat.:
    U1019m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Zinc finger protein 335
    Cat.:
    U1019r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Zinc finger protein 335
    Cat.:
    P1019Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Zinc finger protein 335
    Cat.:
    P1019Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Zinc finger protein 335
    Cat.:
    P1019Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat Zinc finger protein 335
    Monoclonal Antibody for Rat Zinc finger protein 335
    Monoclonal Antibody for Rat Zinc finger protein 335
    Protein for Rat Zinc finger protein 335
    Protein for Rat Zinc finger protein 335
    Protein for Rat Zinc finger protein 335

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.5%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    94-103

    Plasma

    98

    95-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    96-105%

    93-101%

    95-105%

    92-106%

    EDTA plasma(n=5)

    98-105%

    94-105%

    97-106%

    94-106%

    heparin plasma(n=5)

    96-104%

     

    97-110%

    96-111%

    96-109%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
      Submitted (2005-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    2. 2.
      "NRC-interacting factor 1 is a novel cotransducer that interacts with and regulates the activity of the nuclear hormone receptor coactivator NRC."
      Mahajan M.A. , Murray A. , Samuels H.H.
      Mol. Cell. Biol.22:6883-6894(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 514-1336;INTERACTION WITH NCOA6
      strain: Wistar Furth.
      tissue: Pituitary.
    3. 3.
      "Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1."
      Garapaty S. , Mahajan M.A. , Samuels H.H.
      J. Biol. Chem.283:6806-6816(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RQCD1
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